Antioxidant capacity and in vitro bioactive metabolites of the essential oil of Tagetes erecta and Tagetes patula
DOI:
https://doi.org/10.53673/th.v1i1.5Keywords:
chromatography, Density, essential oil, solubilityAbstract
The research had the objective of investigating the bioactive metabolites and antioxidant activity of essential oils of two species of the genus Tagetes. The essential oil was obtained by steam stripping, then the extraction yield, relative density, refractive index and its solubility in ethanol (70% v/v) were determined. The chemical composition was evaluated by gas chromatography coupled to mass spectrometry (GC-MS). The antioxidant activity was determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical method and the radical cation trapping capacity ABTS*+. In the essential oils of the Tagetes species, it was possible to identify 26 chemical components for the Tagetes patula species and 16 for Tagetes erecta. Both species presented monoterpenes (61%) and sesquiterpenes (44%) as main components. The bioactive metabolites of essential oils between both species of Tagetes were β-trans-Ocimene (25.03%), Trans-Tagetone (51.37%), β-Myrcene (2.78) and β-Caryophyllene (1, 17%). The extraction yields ranged between 0.05 and 0.048%, the density between 0.90 and 0.88 (g/ml) with a refractive index of 1.493 and 1.482 and a positive solubility (v/v) between both species. The antioxidant activity of the essential oil in both species showed a variation between 1.77 and 2.56 mg/mL for DPPH and 21.02 to 41.06 mg/mL for BTS*+. The essential oils of the species Tagetes erecta and Tagetes patula are a source of bioactive metabolites that promote antimicrobial and antioxidant potential for use as food preservatives.
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